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青花菜小孢子再生植株加倍及倍性鉴定

d haploids of rape (Brassica napus L.) by in vitro colchicine treatment[J]. Plant Breeding,1991,106(1):82-84.[3] Lichter R. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species[J]. Plant Breeding,1989,103:119-123.[4] Gland A, Lithter R, Schweiger H G. Genetic and exogenous factors affecting embryogenesis in isolated microspore culture of Brassica napus L.[J]. Journal of Plant Physiology,1988,132:613-617.[5] 王秀英,巫东堂,赵军良,等.大白菜游离小孢子培养研究进展[J].蔬菜,2012(7):46-49.[6] Compton M E, Barnett N, Gray D J. Use of fluorescein diacetate (FDA) to determine ploidy of in vitro watermelon shoots[J]. Plant Cell Tissue and Organ Culture,1999,58(3):199-203.[7] 袁素霞,刘玉梅,方智远,等.甘蓝类蔬菜小孢子再生植株染色体倍性与气孔保卫细胞叶绿体数的相关性[J].中国农业科学,2009,42(1):189-197.[8] 张振超,耿鑫鑫,戴忠良,等.甘蓝类植物小孢子培养及植株再生研究[J].核农学报,2013,27(7):0929-0937.[9] 张振超,张蜀宁,张伟,等.四倍体不结球白
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